RT Journal Article
T1 Biological interactions between mercury and selenium in distribution and detoxification processes in mice under controlled exposure. Effects on selenoprotein
A1 García Sevillano, Miguel Ángel
A1 Rodríguez Moro, Gema
A1 García Barrera, Tamara
A1 Navarro, Francisco
A1 Gómez Ariza, José Luis
AB Antagonistic interactions between mercury (Hg) and selenium (Se), were evaluated in mouse (Mus musculus), as a mammalian model, in a series of controlled exposure experiments. The beneficial effect of Se against Hg toxicity involves a variety of biochemical and toxicological processes that have not been clarified yet. For this purpose, a metallomic workflow based on the use of size-exclusion chromatography (SEC) with inductively coupled plasma mass spectrometry (ICP-MS) detection was complemented with the speciation of selenoproteins and low molecular mass selenium species in serum and liver cytosolic extracts using a multidimensional approach based on SEC-AF-HPLC-ICPMS, using species-unspecific isotope dilution (SUID)-ICP-MS for selenium quantification. The results showed potential interactions between Hg/Se in organs and serum related to accumulation and detoxification processes, in addition to the effects of mercury on selenoproteins in hepatic cytosolic extracts and bloodstream when both elements are administrated at the same time. These results provide information about elements distribution, interactions and homeostasis and reveal the potential of metallomic approaches in exposure experiments.
PB Elsevier
SN 0009-2797
SN 1872-7786 (electrónico)
YR 2015
FD 2015
LK http://hdl.handle.net/10272/14593
UL http://hdl.handle.net/10272/14593
LA eng
NO García Sevillano, M.A., Rodríguez Moro, G., García Barrera, T., Navarro, F., Gómez Ariza, J.L.: "Biological interactions between mercury and selenium in distribution and detoxification processes in mice under controlled exposure. Effects on selenoprotein".	Chemico-Biological Interactions. Vol. 229, págs. 82-90, (2015). DOI: 10.1016/j.cbi.2015.02.001
NO This work was supported by the project CTM2012-38720-C03-01 from the Spanish Ministry of Economy and Competitiveness, and by projects P08-FQM-3554 and P09-FQM-4659 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). M.A. Garcia Sevillano thanks the Spanish Ministry of Education for a PhD scholarship. G. Rodriguez-Moro thanks the Ministry of Innovation and Science for a Ph.D. grant.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026