RT Journal Article
T1 Modulation of the gut microbiota and the microbial‑produced gut metabolites by diclofenac exposure and selenium supplementation
A1 Rodríguez Moro, Gema
A1 Cabrera Rubio, Raúl
A1 Selma Royo, Marta
A1 Gómez Morlote, José Antonio
A1 Collado, Maria Carmen
A1 Abril, Nieves
A1 García Barrera, Tamara
AB Diclofenac (DCF) exposure is of great concern due to the ecotoxicological risk linked with a decline of vulture populationsin Southeast Asia, but also because it can affect the reproduction and neurotoxicity in mammals. Otherwise, selenium (Se)is an antioxidant essential element with key roles in health and with antagonistic action against pollutants, but in some caseswith a synergistic effect. To investigate the potential intertwined mechanisms between DCF, Se, and gut microbiota, gutmetabolomic and gut microbiota profiles were determined in mice after DCF exposure and Se supplementation. Speciationof selenoproteins in plasma was carried out by isotopic dilution analysis to quantify the levels of selenoproteins. Significantdifferences in the levels of 79% of the gut metabolites were determined after DCF exposure. The most significant alteredpathway in DCF and DCF-Se groups is the primary bile biosynthesis, being the only pathway altered in mice exposed toDCF, while in DCF-Se, the metabolism of galactose and linoleic acid is also altered. Moreover, specific associations betweenspecific gut microbiota and metabolites were determined in the studied mice groups suggesting intertwined mechanisms.Selenium supplementation modulated the gut metabolic and microbiota profiles affected by DCF.
PB Springer
SN 0944-1344
SN 1614-7499 (electrónico)
YR 2025
FD 2025-03
LK https://hdl.handle.net/10272/25408
UL https://hdl.handle.net/10272/25408
LA eng
NO Rodríguez-Moro, G., Cabrera-Rubio, R., Selma-Royo, M., Gómez-Morlote, J. A., Collado, M. C., Abril, N., & García-Barrera, T. (2025). Modulation of the gut microbiota and the microbial-produced gut metabolites by diclofenac exposure and selenium supplementation. In Environmental Science and Pollution Research. Springer Science and Business Media LLC. https://doi.org/10.1007/s11356-025-36233-6
NO Funding for open access publishing: Universidad de Huelva/CBUA. This work was supported by the projects PID2021-123073NBC21(MICIN/AEI/10.13039/501100011033/FEDER/UE) and PG2018-096608-B-C21 (MCIU/AEI/FEDER, UE) from the Spanish Ministry of Science and Innovation, UHU-202009 (FEDER Andalusian OperativeProgram 2014–2020), and PY20-00366 from the Ministry of Economy,Knowledge, Business and Universities, Regional Government ofAndalusia, Spain. The authors would like to acknowledge the supportfrom The Ramón Areces Foundation (ref. CIVP19A5918). Theauthors are grateful to FEDER (European Community) for financialsupport, Grant UNHU13-1E-1611.G. Rodríguez-Moro thanks PlanAndaluz de Investigación, Desarrollo e Innovación (PAIDI 2020)and Fondo Social Europeo for a postdoctoral grant (DOC_01115).R. Cabrera-Rubio would acknowledge the CDIGENT-GVA attractiontalent program. Funding for open access charge: Universidad deHuelva/CBUA. IATA-CSIC authors also acknowledge the Center ofExcellence Accreditation Severo Ochoa (CEX2021-001189-S/MCIN/AEI/10.13039/501100011033).
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026