@article{10272/18845,
year = {2020},
month = {7},
url = {http://hdl.handle.net/10272/18845},
abstract = {The urinary arsenic metabolites may vary among individuals and the genetic factors
have been reported to explain part of the variation. We assessed the influence of polymorphic
variants of Arsenic-3-methyl-transferase and Glutathione-S-transferase on urinary arsenic metabolites.
Twenty-two groundwater wells for human consumption from municipalities of Colombia were
analyzed for assessed the exposure by lifetime average daily dose (LADD) (μg/kg bw/day). Surveys on
151 participants aged between 18 and 81 years old were applied to collect demographic information and
other factors. In addition, genetic polymorphisms (GSTO2-rs156697, GSTP1-rs1695, As3MT-rs3740400,
GSTT1 and GSTM1) were evaluated by real time and/or conventional PCR. Arsenic metabolites:
AsIII, AsV, monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) were measured using
HPLC-HG-AFS. The influence of polymorphic variants, LADD and other factors were tested using
multivariate analyses. The median of total arsenic concentration in groundwater was of 33.3 μg/L
and the median of LADD for the high exposure dose was 0.33 μg/kg bw/day. Univariate analyses
among arsenic metabolites and genetic polymorphisms showed MMA concentrations higher in
heterozygous and/or homozygous genotypes of As3MT compared to the wild-type genotype. Besides,
DMA concentrations were lower in heterozygous and/or homozygous genotypes of GSTP1 compared
to the wild-type genotype. Both DMA and MMA concentrations were higher in GSTM1-null
genotypes compared to the active genotype. Multivariate analyses showed statistically significant
association among interactions gene-gene and gene-covariates to modify the MMA and DMA
excretion. Interactions between polymorphic variants As3MT*GSTM1 and GSTO2*GSTP1 could
be potential modifiers of urinary excretion of arsenic and covariates as age, LADD, and alcohol
consumption contribute to largely vary the arsenic individual metabolic capacity in exposed people.},
organization = {We wish to thank Matias Martínez from CQF, University of Chile and Sindy Mendoza from
GQYMA, University of Cartagena. This research was supported by Ministerio de Ciencia, Tecnología e Innovación (MinCiencias) in Colombia (Grant No. 110777757778)},
publisher = {MDPI},
title = {As3MT and GST Polymorphisms Influencing Arsenic Metabolism in Human Exposure to Drinking Groundwater},
doi = {10.3390/ijms21144832},
author = {González Martínez, Farith and Sánchez-Rodas Navarro, Daniel Alejandro and Varela, Nelson and Sandoval, Christopher and Quiñones, Luis and Johnson-Restrepo, Boris},
}