RT Journal Article
T1 As3MT and GST Polymorphisms Influencing Arsenic Metabolism in Human Exposure to Drinking Groundwater
A1 González Martínez, Farith
A1 Sánchez-Rodas Navarro, Daniel Alejandro
A1 Varela, Nelson
A1 Sandoval, Christopher
A1 Quiñones, Luis
A1 Johnson-Restrepo, Boris
AB The urinary arsenic metabolites may vary among individuals and the genetic factorshave been reported to explain part of the variation. We assessed the influence of polymorphicvariants of Arsenic-3-methyl-transferase and Glutathione-S-transferase on urinary arsenic metabolites.Twenty-two groundwater wells for human consumption from municipalities of Colombia wereanalyzed for assessed the exposure by lifetime average daily dose (LADD) (μg/kg bw/day). Surveys on151 participants aged between 18 and 81 years old were applied to collect demographic information andother factors. In addition, genetic polymorphisms (GSTO2-rs156697, GSTP1-rs1695, As3MT-rs3740400,GSTT1 and GSTM1) were evaluated by real time and/or conventional PCR. Arsenic metabolites:AsIII, AsV, monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) were measured usingHPLC-HG-AFS. The influence of polymorphic variants, LADD and other factors were tested usingmultivariate analyses. The median of total arsenic concentration in groundwater was of 33.3 μg/Land the median of LADD for the high exposure dose was 0.33 μg/kg bw/day. Univariate analysesamong arsenic metabolites and genetic polymorphisms showed MMA concentrations higher inheterozygous and/or homozygous genotypes of As3MT compared to the wild-type genotype. Besides,DMA concentrations were lower in heterozygous and/or homozygous genotypes of GSTP1 comparedto the wild-type genotype. Both DMA and MMA concentrations were higher in GSTM1-nullgenotypes compared to the active genotype. Multivariate analyses showed statistically significantassociation among interactions gene-gene and gene-covariates to modify the MMA and DMAexcretion. Interactions between polymorphic variants As3MT*GSTM1 and GSTO2*GSTP1 couldbe potential modifiers of urinary excretion of arsenic and covariates as age, LADD, and alcoholconsumption contribute to largely vary the arsenic individual metabolic capacity in exposed people.
PB MDPI
SN 1422-0067
YR 2020
FD 2020-07
LK http://hdl.handle.net/10272/18845
UL http://hdl.handle.net/10272/18845
LA eng
NO González Martínez, F., Sánchez Rodas, D., Varela, N. M., Sandoval, C. A., Quiñones, L. A., & Johnson-Restrepo, B. (2020). As3MT and GST Polymorphisms Influencing Arsenic Metabolism in Human Exposure to Drinking Groundwater. International Journal of Molecular Sciences, 21(14), 4832. DOI:  https://doi.org/10.3390/ijms21144832
NO We wish to thank Matias Martínez from CQF, University of Chile and Sindy Mendoza fromGQYMA, University of Cartagena. This research was supported by Ministerio de Ciencia, Tecnología e Innovación (MinCiencias) in Colombia (Grant No. 110777757778)
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026