RT Journal Article T1 Metabolic and Stress Responses in Senegalese Soles (Solea senegalensis Kaup) Fed Tryptophan Supplements: Effects of Concentration and Feeding Period A1 Herrera Rodríguez, Marcelino A1 Miró, Juan M. A1 Giráldez Díaz, Inmaculada A1 Salamanca de las Nieves, Natalia AB The objective of this study was to assess the impact of di erent dietary Trp concentrations onthe stress and metabolism response of juvenile Senegalese soles (Solea senegalensis). Fish (38.1 1.9 g)were fed di erent Trp-enriched feeds (0%, 1% and 2% Trp added) for two and eight days, and laterexposed to air stress for three min. Samples were taken pre- and 1 h post-stress (condition). Plasmacortisol, lactate, glucose and proteins were significantly a ected by the sampling time, showing highervalues at 1 h post-stress. Trp concentration in food also had significant e ects on lactate and glucoselevels. However, the feeding period did not a ect these parameters. Post-stress values were higherthan in the pre-stress condition for every plasma parameter, except for lactate in two days and 1% Trptreatment. Nevertheless, cortisol, glucose and lactate did not vary significantly between pre- andpost-stress samplings in fish fed the 1% Trp-enriched diet for two days. The lack of variability incortisol response was also due to the high pre-stress value, significantly superior to pre-stress control.The exposure time to Trp feeding did not significantly a ect any enzyme activity; however, Trp addedand condition influenced protein-related enzyme activities. In spite of decreasing stress markers,Trp-enriched diets altered the protein metabolism. PB MDPI SN 2076-2615 YR 2019 FD 2019-06 LK http://hdl.handle.net/10272/16679 UL http://hdl.handle.net/10272/16679 LA eng NO Herrera, M., Miró, J. M., Giráldez, I. ... Salamanca, N. (2019). Metabolic and Stress Responses in Senegalese Soles (Solea senegalensis Kaup) Fed Tryptophan Supplements: Effects of Concentration and Feeding Period. Animals, 9(6), 320. DOI: https://doi.org/10.3390/ani9060320 NO This research was funded by the projects INIA RTA2015-00025-C03-0, and AGL2016-76069-C2-1-R. DS Repositorio Institucional de la Universidad de Huelva RD 1 jun 2026