RT Journal Article T1 Phenylalanine and Tyrosine as Feed Additives for Reducing Stress and EnhancingWelfare in Gilthead Seabream and Meagre A1 Salamanca de las Nieves, Natalia A1 Giráldez Díaz, Inmaculada A1 Morales Carrillo de Albornoz, Emilio A1 Rosa Lucas, Ignacio de la A1 Herrera Rodríguez, Marcelino AB Increased aquaculture production is associated with a growing interest in improving fishwelfare. For this reason, the search for strategies to mitigate stress has intensified, one of thesestrategies being food supplementation with amino acids. The objective of this study was to evaluatethe effects of dietary phenylalanine (Phe) and Tyrosine (Tyr) on the stress response and metabolismof juvenile gilthead seabreams (Sparus aurata) and meagres (Argyrosomus regius). Fish batches werefed a control diet and two diets supplemented with 5% Phe or Tyr for seven days. At the end ofthe experiment fish were stressed by air exposure for 3 min and then sacrificed for the extraction ofblood and brain. Classical plasma stress markers were analyzed (glucose, lactate, proteins, cortisol),as well as hormones derived from those amino acids (adrenaline, noradrenaline and dopamine).Despite interspecific differences, fish fed the diets supplemented with Phe or Tyr showed a reductionon several stress markers. However, interspecific differences were detected for many indicators.Concretely, hormonal stress markers were significantly attenuated in meagres fed the enriched diets.Moreover, the stress condition favored a mobilization of amino acids towards the brain, especiallyin supplemented diets, hence this amino acid excess could be used as an energy substrate to copewith stress. PB MDPI SN 2171-9624 YR 2020 FD 2020-12 LK http://hdl.handle.net/10272/19404 UL http://hdl.handle.net/10272/19404 LA eng NO Salamanca, N., Giráldez Díaz, I., Morales Carrillo de Albornoz, E., Rosa Lucas, I. de la , & Herrera, M. (2020). Phenylalanine and Tyrosine as Feed Additives for Reducing Stress and Enhancing Welfare in Gilthead Seabream and Meagre. Animals, 11(1), 45. DOI: https://doi.org/10.3390/ani11010045 DS Repositorio Institucional de la Universidad de Huelva RD 31 may 2026