@article{10272/27991, year = {2026}, url = {https://hdl.handle.net/10272/27991}, abstract = {This study evaluated the bioaccessibility of carotenoids from wild-type and phytoene-enriched Dunaliella bardawil biomass treated by ultrasounds and ball-milling. Fresh, freeze-dried, and alginate-encapsulated matrices were studied. The impact of yogurt addition was also evaluated on freeze-dried samples. Carotenoid bioaccessibility varied significantly depending on the matrix, treatment, and carotenoid isomer. As an example, ballmilling at 30 Hz was the treatment leading to the highest bioaccessibility in fresh samples (p < 0.05), whereas this treatment at 5 Hz was optimal for freeze-dried matrices, with no significant differences with the control in the wild-type matrix. The addition of yogurt enhanced carotenoid bioaccessibility (p < 0.05) in both freeze-dried matrices. Xanthophylls showed slightly higher bioaccessibility than carotenes, and cis-isomers outperformed their all-trans counterparts. (15Z)-Phytoene and (all-E)-lutein showed the greatest bioaccessibility in most treatments. These results can help improve the bioavailability of carotenoids from D. bardawil through optimized pretreatment strategies.}, organization = {This study was financially supported by grants PID2019-110438RB- C21 (NEWCARFOODS) and PID2019-110438RB-C22 (BESTALGAE4 CAROT), funded by MCIN/AEI/ 10.13039/501100011033. PMB grate fully acknowledge funding from the VII Plan Propio de Investigación y Transferencia of the University of Seville (VII PPIT-US), under the Contrato Acción II.4 PPIT (Plan de Captación y Retención de Talento). AMO was supported by a grant associated to grant PID2019-110438RB- C21 (NEWCARFOODS). AMO, AMBG, PMB and AJMM are members of the Spanish Carotenoid Network (CaRed), grant RED2022-134577-T, funded by MCIN/AEI/10.13039/501100011033.}, publisher = {Elsevier}, title = {Effect of ultrasounds and ball milling on the bioaccessibility of carotenoids from the microalga Dunaliella bardawil}, doi = {10.1016/j.foodchem.2025.147332}, author = {Morón Ortiz, Ángeles and Benítez González, Ana M. and León Vaz, Antonio and León Bañares, Rosa María and Mapelli Brahm, Paula and Meléndez Martínez, Antonio J.}, }