RT Journal Article T1 Composted sewage sludge as an alternative substrate for forest seedlings production A1 Gabira, Mônica Moreno A1 Silva, Richardson Barbosa Gomes da A1 Bortolheiro, Fernanda Pacheco de Almeida Prado A1 Mateus, Caroline de Moura D’Andrea A1 Villas Boas, Roberto Lyra A1 Rossi, Sergio A1 Montoro Girona, Miguel A1 Silva, Magali Ribeiro da AB The production of forest seedlings with adequate morphological and physiological characteristics is essential for the success of plantations. Substrates and irrigation are the major factors determining seedlings’ growth. Substrates made of urban and agricultural residues are a sustainable alternative to peat-based substrates. In this study, we evaluated how composted sewage sludge substrates affect the growth and gas exchange in seedlings of Cedrela fissilis Vell. Seedlings were produced under daily irrigation depths of 6, 9, and 12 mm, and on different substrates. The substrates were based on sewage sludge composted with Eucalyptus bark or sugarcane bagasse, and a commercial substrate based on peat, involving a double factorial design with 12 treatments (3 irrigation depths × 3 substrates). Both physical and chemical characteristics of substrates were analyzed, and morphological traits and gas exchanges of seedlings were measured. Sewage sludge-based substrates presented different characteristics according to the material it was mixed. Eucalyptus bark provided higher bulk density (0.19 g cm-3) and lower total porosity (75%) to the substrate, while sugarcane bagasse increased macroporosity up to 60%. Seedlings produced in sewage sludge-based substrates presented a height up to 17.8 cm and stem diameters of between 8.39-10.29 mm. Higher shoot and root dry mass was obtained in sewage sludge-based substrates with irrigation depth of 9 mm, which were 3.71 and 2.01 g, respectively. Photosynthetic carbon assimilation varied between 2.26 and 3.23 µmol CO2 m-2 s-1, and water use efficiency varied from 2.058 to 3.395 µmol CO2 (mol H2O)-1, with the highest values being obtained in seedlings produced in sewage sludge-based substrates with irrigation depth of 6 mm. Our results demonstrate that sewage sludge-based substrates are an efficient alternative to commercial peat-based substrates for seedling production. PB Italian Society of Silviculture and Forest Ecology SN 1971-7458 YR 2021 FD 2021 LK http://hdl.handle.net/10272/21206 UL http://hdl.handle.net/10272/21206 LA eng NO Gabira, M., Silva, R., Bortolheiro, F., Mateus, C., Villas Boas, R., Rossi, S., Girona, M., & Silva, M. (2021). Composted sewage sludge as an alternative substrate for forest seedlings production. In iForest - Biogeosciences and Forestry (Vol. 14, Issue 6, pp. 569–575). Italian Society of Sivilculture and Forest Ecology (SISEF). https://doi.org/10.3832/ifor3929-014 DS Repositorio Institucional de la Universidad de Huelva RD 1 jun 2026