RT Journal Article
T1 Antagonistic interaction of selenomethionine enantiomers on methylmercury toxicity in the microalgae Chlorella sorokiniana
A1 Moreno Roldán, Fernando
A1 García Barrera, Tamara
A1 Gómez Jacinto, Verónica
A1 Gómez Ariza, José Luis
A1 Garbayo Nores, Inés
A1 Vílchez Lobato, Carlos
AB The protective effect of selenium against mercury toxicity is well known especially between selenomethionine and methylmercury and it has been studied in several living organisms, however information is lacking about the interaction of these species in Chlorella. Investigation into which chiral form of selenomethionine effectively acts against the toxic effects of methylmercury has not previously been carried out. In the present work, two control cultures and two cultures of C. sorokiniana were grown in standard medium with D,L-SeMet, L-SeMet or D-SeMet. After the experiment was started up MeHg+ was added periodically to the cultures containing D,L-SeMet, L-SeMet, D-SeMet and to one of the control batches. The results show that both SeMet enantiomers counteract the toxicity of MeHg+, by markedly increasing the total content of chlorophyll, carotenoids, as well as the dry weight and light dependent oxygen production, compared to the culture which is non pre-treated with SeMet and is only exposed to MeHg+. The levels of MeHg+ measured in cells are lower in the cultures pre-treated with SeMet indicating that the passage of MeHg+ into the cells is negligible when carried out in the presence of SeMet, or that SeMet enhances the release of MeHg+. On the other hand, L-SeMet is directly involved in the detoxification of MeHg+, but the involvement of D-SeMet occurs only indirectly since it has been neither identified in the medium nor in C. sorokiniana after supplementation with this enantiomer. It may be that D-SeMet is transformed into SeMeSec and L-SeMet. Moreover, SeMeSec is almost totally released from the cells after 72 hours. No mercury–selenium complex was detected but, since the summation of the different species identified accounted only for 77% of the total selenium and mercury measured directly after sample digestion, it is possible that they are present in the form of an undetected Se–Hg complex. This hypothesis is supported by the decrease of inorganic selenium during the experiment. The present paper reports new data about the relationship between the mechanism of detoxification of methylmercury and selenomethionine enantiomers through the study of the metabolic intermediates by means of speciation analysis.
PB Royal Society of Chemistry
SN 1756-5901
SN 1756-591X (electrónico)
YR 2014
FD 2014
LK http://hdl.handle.net/10272/14731
UL http://hdl.handle.net/10272/14731
LA eng
NO Moreno, F., García-Barrera, T., Gómez-Jacinto, V., Gómez-Ariza, J. L., Garbayo-Nores, I., & Vílchez-Lobato, C. (2014). Antagonistic interaction of selenomethionine enantiomers on methylmercury toxicity in the microalgae Chlorella sorokiniana. Metallomics, 6(2), 347. https://doi.org/10.1039/c3mt00296a
NO The work was supported by "Ministerio de Educacion y Ciencia (MEC)'' [CTM2006-08960-C02-01] and "Consejeria de Innovacion Ciencia y Empresa (Junta de Andaluci a)'' [projects P08-FQM-03554 and P09-FQM-4659]. F. Moreno is grateful to the "Ministerio de Educacion y Ciencia (MEC)'' for a PhD grant [CTM2006-08960-C02-01/TECNO].
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026