RT Journal Article T1 Search for optimum conditions of wheat straw hemicelluloses cold alkaline extraction process A1 García Domínguez, Juan Carlos A1 Díaz Blanco, Manuel Jesús A1 García Domínguez, María Trinidad A1 Feria Infante, Manuel Javier A1 Gómez Lozano, D. M. A1 López Baldovín, Francisco AB A method for the selective extraction of hemicellulose from wheat straw involving cold alkaline extractionand subsequent separation by precipitation with ethanol is proposed. Wheat straw affords selectiveseparation of the hemicellulose fraction from the cellulose and lignin fractions with the proposed method.The hemicellulose yield was optimized by using a 2n factor design to examine the influence of temperatures(temperature was designed between 20 and 40 ◦C), operation times (operation time was designedbetween 30 and 60 min) and alkali concentrations (alkali concentration was designed between 80 and120 g L−1). These conditions allowed 56.1% of all hemicellulose initially present in the raw material, and59.1% of the lignin, to be extracted. Subsequent separation of hemicellulose in the liquid phase from thecold alkaline extraction by precipitation with ethanol provided a fraction containing 39.4% of all hemicellulose(45.2% hemicellulose in extract/total extract) and only 12% of all lignin in the raw material. PB Elsevier SN 1369-703X YR 2013 FD 2013 LK http://hdl.handle.net/10272/10358 UL http://hdl.handle.net/10272/10358 LA eng NO García Domínguez, J.C., Díaz Blanco, M.J., García Domínguez, M.T., Feria Infante, M.J., Gómez Lozano, D.M.:"Search for optimum conditions of wheat straw hemicelluloses cold alkaline extraction process". Biochemical Engineering Journal. Vol. 71, págs. 127-133, (2013). ISSN 1369-703X NO The authors are grateful for the FPU grant from the Spanish Ministry of Education. Also they thank to Spanish Ministry of Economy and Competitiveness (former Ministry of Science and Innovation) contracts. The authors acknowledge financial support for this investigation has been provided by CDTI (CENIT-E-CDTI-BioSos-CEN-2009-1040) and supported by Ministry of Economy and Competitiveness, "Local Investment fund for employment". Government of Spain, Junta de Andalucia, CICYT-FEDER (Science and Technology Inter Ministerial Commission, Spanish Government - European Regional Development Fund), project number AGL2009-13113 and the business group ACCIONA INFRAESTRUCTURAS, S.A. DS Repositorio Institucional de la Universidad de Huelva RD 1 jun 2026