RT Journal Article T1 Does the Age of Pinus sylvestris Mother Trees Influence Reproductive Capacity and Offspring Seedling Survival? A1 Pardos, Marta A1 Vázquez Piqué, Javier A1 Benito, Luis A1 Madrigal, Guillermo A1 Alejano Monge, Reyes A1 Fernández Martínez, Manuel A1 Calama, Rafael AB We assess how the age of Pinus sylvestris mother trees influences seed size, seed viability,germination capacity and later offspring seedling survival under greenhouse conditions. Thirty treesranging from 30 to 219 years old were selected in the north facing slopes in the Sierra de Guadarrama,where we could find the oldest Pinus sylvestris trees in central Spain. Forty cones per tree wereharvested to study cone and seed characteristics (size and weight), seed viability and germinationcapacity related to the mother tree age. In addition, 25 germinated seeds per tree were grown in agreenhouse to assess offspring seedling survival during a death trial, where watering was stopped.Significant differences between trees in cone and seed morphological traits were observed. The ageof the mother tree had a significant effect on cone size, seed size, and seed weight, but there wasno effect on seed germination capacity and seed viability. Seedling survival was mainly affectedby the decrease in water availability. However, a significant effect of the tree age was found oncesoil moisture had reached 0%. Our results show the ability of overmature Pinus sylvestris trees tomaintain a relatively high reproductive capacity that assures its persistence PB MDPI SN 1999-4907 (electrónico) YR 2022 FD 2022-06 LK http://hdl.handle.net/10272/21079 UL http://hdl.handle.net/10272/21079 LA eng NO Pardos, M., Vázquez-Piqué, J., Benito, L., Madrigal, G., Alejano, R., Fernández, M., & Calama, R. (2022). Does the Age of Pinus sylvestris Mother Trees Influence Reproductive Capacity and Offspring Seedling Survival? In Forests (Vol. 13, Issue 6, p. 937). MDPI AG. https://doi.org/10.3390/f13060937 NO This research was funded by Ministerio de Ciencia e Innovación, grant number AGL2017-83828-C2. We thank the Forest Service at Sierra de Guadarrama National Park for providingthe sampling permits required for the area and for their interest in the project and Giorgia Fiorino forhelping in the seed viability and seed germination tests DS Repositorio Institucional de la Universidad de Huelva RD 31 may 2026