RT Journal Article
T1 Methodological Optimization of Supercritical Fluid Extraction of Valuable Bioactive Compounds from the Acidophilic Microalga Coccomyxa onubensis
A1 Ruiz Domínguez, María del Carmen
A1 Medina, Elena
A1 Salinas, Francisca
A1 Bugueño, Waldo
A1 Fuentes Cordero, Juan Luis
A1 Vílchez Lobato, Carlos
A1 Garbayo Nores, Inés
A1 Cerezal, Pedro
A1 Ruiz Domínguez, María del Carmen
AB Microalgae grow in diverse environments and possess a great biotechnological potential asthey contain useful bioactive compounds. These bioactive compounds can be obtained by selectiveand energy-efficient extraction methods. Various industries are using the supercritical fluid extraction(SFE) method to extract these valuable bioactive compounds. Hence, for the first time, we evaluatedthe effects of SFE on the recovery of bioactive and antioxidant compounds using Coccomyxa onubensis,a eukaryotic acidophilic microalga of potential relevance which can be used in the field of nutraceuticaland functional foods. It was isolated from the Tinto River (Pyritic Belt, Huelva, Spain), a miningregion in Spain. Variables such as extraction yield, lutein purity (LP) and recovery (LR), total phenols,and antioxidant capacity (Trolox equivalents antioxidant capacity method) were studied using aBox–Behnken design based on a response surface methodology along with the overall extractioncurve fitted to a spline linear model. The effects of temperature (30, 50, and 70  C), pressure (25, 40,and 55 MPa), and the percentage of co-solvent (0, 25%, and 50% v/v ethanol) on SFE were analyzed,resulting in the co-solvent and temperature as the most significant factors followed by the pressure.Under 70  C, 40 MPa, and 50% v/v ethanol, C. onubensis reached a maximum of 66.98% of LR.The extracts were richest in total phenols and showed the maximum antioxidant activity (36.08 mgGAEs/g extracts and 2.237 mmol TE/g extracts, respectively) under similar pressure and co-solventpercentage values and different temperatures (30 and 70  C, respectively). The extracts obtained inthis study may have potential applications in the food, nutraceutical, and cosmetic industries. SFE isa highly efficient method to valorize microorganisms living in extreme environments, which are sofar unexplored using green extraction methods
PB MDPI
SN 2076-3921 (electrónico)
YR 2022
FD 2022-06
LK http://hdl.handle.net/10272/21070
UL http://hdl.handle.net/10272/21070
LA eng
NO Ruiz-Domínguez, M. C., Medina, E., Salinas, F., Bugueño, W., Fuentes, J.-L., Vílchez, C., Garbayo, I., & Cerezal-Mezquita, P. (2022). Methodological Optimization of Supercritical Fluid Extraction of Valuable Bioactive Compounds from the Acidophilic Microalga Coccomyxa onubensis. In Antioxidants (Vol. 11, Issue 7, p. 1248). MDPI AG. https://doi.org/10.3390/antiox11071248
NO This work was partially supported by the grant FONDEQUIP EQM-160073 (public fundsof Chile-ANID), by the Andalusian Research, Development and Innovation Plan (Junta de Andalucía,Spain), FEDER funds (Project P20_00930), and by the University of Huelva through a grant obtainedin the program “Requalitication of the Spanish Universitary System 2021–2023, María Zambrano”(Real Decreto 289/2021, 20 April and Order UNI/551/2021, 26 May). The research group “LAMICBA” at the University of Antofagasta, Chile, thanksthe “BITAL group” at the University of Huelva (Spain) for providing microalgae samples
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026