RT Journal Article
T1 Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital
A1 Molina Delgado, Lázaro
A1 Udaondo, Zulema
A1 Duque, Estrella
A1 Molina Santiago, Carlos
AB Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the resistanceprofiles of animal and human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil andwater but have been seldom isolated from humans. We have established a collection of P. putida strains isolated from inpatientsin different hospitals in France. One of the isolated strains (HB3267) kills insects and is resistant to the majority ofthe antibiotics used in laboratories and hospitals, including aminoglycosides, ß-lactams, cationic peptides, chromoproteinenediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones and quinolones, glycopeptide antibiotics,macrolides, polyketides and sulfonamides. Similar to other P. putida clinical isolates the strain was sensitive to amikacin. Toshed light on the broad pattern of antibiotic resistance, which is rarely found in clinical isolates of this species, the genomeof this strain was sequenced and analysed. The study revealed that the determinants of multiple resistance are bothchromosomally-borne as well as located on the pPC9 plasmid. Further analysis indicated that pPC9 has recruited antibioticand biocide resistance genes from environmental microorganisms as well as from opportunistic and true human pathogens.The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreadingantibiotic resistant determinants to new hosts.
PB Public Library of Science
SN 1932-6203
YR 2014
FD 2014-01
LK http://hdl.handle.net/10272/18353
UL http://hdl.handle.net/10272/18353
LA eng
NO Molina Delgado, L., Udaondo, Z., Duque, E. ...  Molina Santiago, C. (2014). Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital. PLoS ONE, 9(1), e81604. DOI: https://doi.org/10.1371/journal.pone.0081604
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026