RT Journal Article
T1 Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture
A1 Blázquez Carmona, Pablo
A1 Ruiz Mateos, Raquel
A1 Barrasa Fano, Jorge
A1 Shapeti, Apeksha
A1 Martín Alfonso, José Enrique
A1 Domínguez Abascal, Jaime
A1 Van Oosterwyck, Hans
A1 Reina Romo, Esther
A1 Sanz Herrera, José A.
AB Collagen-based hydrogels are commonly used in mechanobiology to mimic the extracellular matrix. A quantitative analysis of the influence of collagen concentration and properties on the structure and me- chanics of the hydrogels is essential for tailored design adjustments for specific in vitro conditions. We combined focused ion beam scanning electron microscopy and rheology to provide a detailed quanti- tative atlas of the mechanical and nanoscale three-dimensional structural alterations that occur when manipulating different hydrogel’s physicochemistry. Moreover, we study the effects of such alterations on the phenotype of breast cancer cells and their mechanical interactions with the extracellular matrix. Regardless of the microenvironment’s pore size, porosity or mechanical properties, cancer cells are able to reach a stable mesenchymal-like morphology. Additionally, employing 3D traction force microscopy, a positive correlation between cellular tractions and ECM mechanics is observed up to a critical threshold, beyond which tractions plateau. This suggests that cancer cells in a stable mesenchymal state calibrate their mechanical interactions with the ECM to keep their migration and invasiveness capacities unaltered.
PB Elsevier
SN 1742-7061
SN 1878-7568 (electrónico)
YR 2024
FD 2024-07
LK https://hdl.handle.net/10272/24759
UL https://hdl.handle.net/10272/24759
LA eng
NO Blázquez-Carmona, P., Ruiz-Mateos, R., Barrasa-Fano, J., Shapeti, A., Martín-Alfonso, J. E., Domínguez, J., Van Oosterwyck, H., Reina-Romo, E., & Sanz-Herrera, J. A. (2024). Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture. In Acta Biomaterialia (Vol. 185, pp. 281–295). Elsevier BV. https://doi.org/10.1016/j.actbio.2024.07.002
NO This work was supported by MCIN/AEI/ 10.13039/50110 0 011033 [ PID2020-113790RB-I00, PID2021-126051OB-C42 ].
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026