RT Unpublished Material
T1 Effect of abiotic stress on the production on lutein and beta-carotene by Chlamydomonas acidophila
A1 Vílchez Lobato, Carlos
A1 Cuaresma Franco, María
A1 Garbayo Nores, Inés
A1 Vega Pérez, José Manuel
AB Chlamydomonas acidophila growing autotrophically with continuous PAR light (160 µE.m-2.s-1) and 30 ºC may accumulate carotenoids which increase in response to abiotic stress, like high light intensity, UV-A radiation and temperature fluctuation. At 240 µE.m-2.s-1 the alga contains 57.5 ± 1.6 mg.l-1 of total carotenoids after 20 days of growing, which does not significantly change by an irradiance of 1000 µE.m-2.s-1. Lutein (20 ± 0.5 mg.l-1) and β-carotene (8.3 ± 0.2 mg.l-1) production were particularly high in C. acidophila, while zeaxanthine (0.2 ± 0.1 mg.l-1) was low. Enhanced production of these carotenoids was also observed in cultures illuminated with PAR light (160 µE.m-2.s-1) supplemented with moderate UV-A radiation (10 µE.m-2.s-1).Optimum algae growth takes place at 40 ºC, like the maximum amount of intracellular lutein and β-carotene. On the other hand, the presence of iron in the culture medium, in a range between 5-35 mM, significantly decreased the cell viability and the intracellular content of carotenoids, however cupper, at 1-5 mM, appears to increase the synthesis of β-carotene. The alga can growth under mixotrophic conditions, with glucose or acetate, 10 mM, as carbon source, but such conditions did not improved the intracellular content of carotenoids.
SN 1359-5113
YR 2008
FD 2008
LK http://hdl.handle.net/10272/2803
UL http://hdl.handle.net/10272/2803
LA eng
NO VÍLCHEZ LOBATO, Carlos; CUARESMA FRANCO, María; GARBAYO NORES, Inés; VEGA PÉREZ, José Manuel. "Effect of abiotic stress on the production on lutein and beta-carotene by Chlamydomonas acidophila". Process Biochemistry. Vol. 43, no 10 (Octubre 2008) p. 1158-1161. ISSN 1359-5113
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026