RT Journal Article T1 Odiel River (SW Spain), a Singular Scenario Affected by Acid Mine Drainage (AMD): Graphical and Statistical Models to Assess Diatoms and Water Hydrogeochemistry Interactions A1 Grande Gil, José Antonio A1 Luís, Ana Teresa A1 Córdoba García, Francisco A1 Leiva, Mercedes A1 Dávila Martín, José Miguel A1 Fortes Garrido, Juan Carlos A1 Santisteban Fernández, María A1 Ferreira da Silva, Eduardo A1 Miguel Sarmiento, Aguasanta AB The Odiel River (SW Spain) is one of the most cited rivers in the scientific literature due to itshigh pollution degree, generated by more than 80 sulphide mines’ (mostly unrestored) contaminationin the Iberian Pyritic Belt (IPB), that have been exploited for more than 5000 years. Along the riverand its tributaries, the physico-chemical parameters and diatoms, from 15 sampling points, wereanalyzed in the laboratory. Physico-chemical parameters, water chemical analysis, together withrichness and Shannon–Wiener indexes were integrated in a matrix. An initial graphical treatmentallowed the definition and proposal of a functioning system model, as well as the establishment ofcause–effect relationships between pollution and its effects on biota. Then, the proposed model wasstatistically validated by factor analysis. For acidic pH waters, high values of Eh, TDS, sulphate,∑REE and ∑Ficklin were found, while diatomologic indicators took low values. Thus, factor analysiswas a very effective tool for graphical treatment validation as well as for pollution–biota interactionmodels’ formulation, governed by two factors: AMD processes and water balance suffered by thestudied river. As a novelty, the cause–effect relationships between high barium concentration andlow diversity and richness were demonstrated in the IPB, for the first time PB MDPI SN 1660-4601 (electrónico) YR 2021 FD 2021 LK http://hdl.handle.net/10272/20277 UL http://hdl.handle.net/10272/20277 LA eng NO Grande, J. A., Luís, A. T., Córdoba, F., Leiva, M., Dávila, J. M., Fortes, J. C., Santisteban, M., Ferreira da Silva, E., & Sarmiento, A. M. (2021). Odiel River (SW Spain), a Singular Scenario Affected by Acid Mine Drainage (AMD): Graphical and Statistical Models to Assess Diatoms and Water Hydrogeochemistry Interactions. In International Journal of Environmental Research and Public Health (Vol. 18, Issue 16, p. 8454). MDPI AG. https://doi.org/10.3390/ijerph18168454 NO : The authors are grateful to the Departments of Biology and Geosciences of theUniversity of Aveiro, Portugal where diatom samples were identified. The authors are grateful to theSustainable Mining Engineering Research Group, Department of Mining, Mechanic, Energetic andConstruction Engineering at the Higher Technical School of Engineering, University of Huelva, Spainfor paying for the water analyses. AT Luís is funded by national funds (OE), through FCT—Fundaçãopara a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5and 6 of the article 23, of the Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026