RT Journal Article
T1 Metabolomic study of lipids in serum for biomarker discovery in Alzheimer's disease using direct infusion mass spectrometry
A1 González Domínguez, Raúl
A1 García Barrera, Tamara
A1 Gómez Ariza, José Luis
AB In this study, we demonstrated the potential of direct infusion mass spectrometry for the lipidomic characterization of Alzheimer's disease. Serum samples were extracted for lipids recovery, and directly analyzed using an electrospray source. Metabolomic fingerprints were subjected to multivariate analysis in order to discriminate between groups of patients and healthy controls, and then some key-compounds were identified as possible markers of Alzheimer's disease. Major differences were found in lipids, although some low molecular weight metabolites also showed significant changes. Thus, important metabolic pathways involved in neurodegeneration could be studied on the basis of these perturbations, such as membrane breakdown (phospholipids and diacylglycerols), oxidative stress (prostaglandins, imidazole and histidine), alterations in neurotransmission systems (oleamide and putrescine) and hyperammonaemia (guanidine and arginine). Moreover, it is noteworthy that some of these potential biomarkers have not been previously described for Alzheimer's disease.
PB Elsevier
SN 0731-7085
SN 1873-264X (electrónico)
YR 2014
FD 2014
LK http://hdl.handle.net/10272/14510
UL http://hdl.handle.net/10272/14510
LA eng
NO González Domínguez, R., García Barrera, T., Gómez Ariza, J.L.: "Metabolomic study of lipids in serum for biomarker discovery in Alzheimer's disease using direct infusion mass spectrometry". Journal of Pharmaceutical and Biomedical Analysis.  Vol. 98, págs.  321-326, (2014). DOI: 10.1016/j.jpba.2014.05.023
NO This work was supported by the projects CTM2012-38720-C03-01 from the Ministerio de Ciencia e Innovacion and P008-FQM-3554 and P009-FQM-4659 from the Consejeria de Innovacion, Ciencia y Empresa (Junta de Andalucia). Raul Gonzalez Dominguez thanks the Ministerio de Educacion for a predoctoral scholarship. The authors also thank to Dr. Alberto Blanco and Carlos Salgado from Hospital Juan Ramon Jimenez for providing serum samples.
DS Repositorio Institucional de la Universidad de Huelva
RD 13 jun 2026