RT Journal Article
T1 Time-Dependent Collagen Fibered Structure in the Early Distraction Callus: Imaging Characterization and Mathematical Modeling
A1 Blázquez Carmona, Pablo
A1 Sanz Herrera, José A.
A1 Mora Macías, Juan
A1 Morgaz, Juan
A1 Domínguez, Jaime
A1 Reina Romo, Esther
AB Collagen is a ubiquitous protein present in regenerating bone tissues that experiences multiple biological phenomena during distraction osteogenesis until the deposition of phosphate crystals. This work combines fluorescence techniques and mathematical modeling to shed light on the mechano-structural processes behind the maturation and accommodation-to-mineralization of the callus tissue. Ovine metatarsal bone calluses were analyzed through confocal images at different stages of the early distraction osteogenesis process, quantifying the fiber orientation distribution and mean intensity as fiber density measure. Likewise, a mathematical model based on the experimental data was defined to micromechanically characterize the apparent stiffening of the tissue within the distracted callus. A reorganization of the fibers around the distraction axis and increased fiber density were found as the bone fragments were gradually separated. Given the degree of significance between the mathematical model and previous in vivo data, reorganization, densification, and bundle maturation phenomena seem to explain the apparent mechanical maturation observed in the tissue theoretically.
PB Springer
SN 0090-6964
SN 1573-9686 (electrónico)
YR 2022
FD 2022
LK https://hdl.handle.net/10272/21727
UL https://hdl.handle.net/10272/21727
LA eng
NO Blázquez-Carmona, P., Sanz-Herrera, J. A., Mora-Macías, J., Morgaz, J., Domínguez, J., & Reina-Romo, E. (2022). Time-Dependent Collagen Fibered Structure in the Early Distraction Callus: Imaging Characterization and Mathematical Modeling. In Annals of Biomedical Engineering (Vol. 50, Issue 12, pp. 1798–1809). Springer Science and Business Media LLC. https://doi.org/10.1007/s10439-022-02992-3
NO This work was supported by the Spanish Ministry ofEconomy, Industry and Competitiveness [Grant No.DPI2017-82501-P]; the Spanish State Research Agency(AEI) [Grant No. PID2020-113790RB-I00/AEI/10.13039/501100011033]; and the European RegionalDevelopment Fund (FEDER) with the Junta de Andalucı´a [Grant No. US-1261691].
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026