RT Journal Article
T1 Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis
A1 Cordero, Baldo F.
A1 Obraztsova, Irina
A1 Couso Liañez, Inmaculada
A1 León Bañares, Rosa María
AB Chlorella sorokiniana has been selected for lutein production, after a screeningof thirteen species of microalgae, since it showed both a high content in this carotenoid anda high growth rate. The effects of several nutritional and environmental factors on cellgrowth and lutein accumulation have been studied. Maximal specific growth rate and luteincontent were attained at 690 μmol photons m−2 s−1, 28 °C, 2 mM NaCl, 40 mM nitrate andunder mixotrophic conditions. In general, optimal conditions for the growth of this strainalso lead to maximal lutein productivity. High lutein yielding mutants of C. sorokinianahave been obtained by random mutagenesis, using N-methyl-N′-nitro-nitrosoguanidine(MNNG) as a mutagen and selecting mutants by their resistance to the inhibitors of thecarotenogenic pathway nicotine and norflurazon. Among the mutants resistant to theherbicides, those exhibiting both high content in lutein and high growth rate were chosen.Several mutants exhibited higher contents in this carotenoid than the wild type, showing, inaddition, either a similar or higher growth rate than the latter strain. The mutant MR-16exhibited a 2.0-fold higher volumetric lutein content than that of the wild type, attainingvalues of 42.0 mg L−1 and mutants DMR-5 and DMR-8 attained a lutein cellular content of7.0 mg g−1 dry weight. The high lutein yield exhibited by C. sorokiniana makes thismicroalga an excellent candidate for the production of this commercially interesting pigment.
PB MDPI
SN 1660-3397
YR 2011
FD 2011-09
LK http://hdl.handle.net/10272/18229
UL http://hdl.handle.net/10272/18229
LA eng
NO Cordero, B. F., Obraztsova, I., Couso Liañez, I., ...  León Bañares, R. (2011). Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis. Marine Drugs, 9(9), 1607–1624. DOI: https://doi.org/10.3390/md9091607
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026