RT Journal Article
T1 D-amino acid peptides as antimicrobial agents against vibrio-associated diseases in aquaculture
A1 López Sanmartín, Montserrat
A1 Rengel Domínguez, Rocío
A1 López López, Manuel
A1 Lebrón, José Antonio
A1 Molina Márquez, Ana María
A1 Rosa Lucas, Ignacio de la
A1 López Cornejo, Pilar
A1 Cuesta, Alberto
A1 Vigara Fernández, Javier
A1 León Bañares, Rosa María
AB Vibriosis is one of the most usual infection diseases in bivalve mollusks, particularly affecting seeds and larvae,which are more susceptible than adults to these bacterial infections. The devastating effect of vibriosis in shellfishhatcheries is aggravated by the increasing resistance of many Vibrio species to traditional antibiotics, highlightingthe need to find new antimicrobial agents. Antimicrobial peptides (AMPs) are a promising alternative,however, their low stability can be a handicap for their practical application. In this study, the 3D structure andthe stability of a synthetic all-D-amino acid peptide (D-Caerin) to proteases and extreme pH conditions have beenanalyzed and compared with its corresponding natural L-enantiomer peptide, Caerin 1.1. Moreover, the antimicrobialactivity of D-Caerin has been tested in vitro against the control bacteria Micrococcus luteus CECT 245;and four Vibrio species: Vibrio aestuarianus CECT 625 T, Vibrio anguillarum CECT 522 T, Vibrio harveyi CECT 525 Tand Vibrio tapetis CECT 4600 T, which are among the most representative causative agents of vibriosis inaquaculture. Our results demonstrate that D-Caerin contains two left-handed alpha helices, and is more stableand effective against the bacterial species tested than its corresponding natural L- counterpart. The bioactivity ofD-Caerin has also been tested in vivo, in clam seeds infected with a mixed inoculum of these vibrio species. Thesepreliminary assays show that D-Caerin-treated specimens exhibit higher survival rates after infection, demonstratingthe superior stability of D-Caerin and its effectiveness against vibriosis-causative agents.
PB Elsevier
SN 0044-8486
YR 2023
FD 2023
LK https://hdl.handle.net/10272/22167
UL https://hdl.handle.net/10272/22167
LA eng
NO López-Sanmartín, M., Rengel, R., López-López, M., Lebrón, J. A., Molina-Márquez, A., de la Rosa, I., López-Cornejo, P., Cuesta, A., Vigara, J., & León, R. (2023). D-amino acid peptides as antimicrobial agents against vibrio-associated diseases in aquaculture. In Aquaculture (Vol. 569, p. 739362). Elsevier BV. https://doi.org/10.1016/j.aquaculture.2023.739362
NO This research was funded by the Andalusian government (I+D+i-JAPAIDI-Retos projects 2020-PY20_00728)
DS Repositorio Institucional de la Universidad de Huelva
RD 30 may 2026