RT Journal Article
T1 Molecular Engineering for Nonlinear Fluorescence: En Route to Three-Photon Absorption via Sequential One-Photon Excitation
A1 Oh, Jinyoung
A1 Benítez Martín, Carlos
A1 Fron, Eduard
A1 Hofkens, Johan
A1 Pischel, Uwe
A1 Grøtli, Morten
A1 Andréasson, Joakim
AB Multiphoton excitation (MPE) processes enable three-dimensionally confined fluorescence with reduced background as well as improved image contrast and signal-to-noise ratio. However, MPE finds major technological limitations derived from the need for high light intensities at long excitation wavelengths. To circumvent these challenges, we herein propose a molecular strategy that reproduces multiphoton-like nonlinear responses using only sequential one-photon excitations (1PE). A dyad (2for1), consisting of the acedan fluorophore covalently connected to a spironaphthopyran photoswitch, shows a quadratic dependence of the emission intensity on the excitation intensity, thus emulating a two-photon absorption behavior. The incorporation of a BODIPY photocage to this construct yields a triad (3for1) that results in an even stronger nonlinear fluorescence response. These findings pave the way for sequential 1PE as a practical approach to capitalize on the benefits of nonlinear fluorescence while avoiding the inherent limitations of simultaneous MPE.
PB American Chemical Society
SN 0002-7863
SN 1520-5126 (electrónico)
YR 2026
FD 2026
LK https://hdl.handle.net/10272/28494
UL https://hdl.handle.net/10272/28494
LA eng
NO Oh, J., Benítez-Martín, C., Fron, E., Hofkens, J., Pischel, U., Grøtli, M., & Andréasson, J. (2026). Molecular Engineering for Nonlinear Fluorescence: En Route to Three-Photon Absorption via Sequential One-Photon Excitation. Journal of the American Chemical Society, 148(20), 20813–20820. https://doi.org/10.1021/jacs.6c03621
NO The authors are grateful for the financial support from the European Innovation Council and SMEs Executive Agency (EISMEA) for the EIC PATHFINDER project 101098934−4 for 2. J. A. thanks the Swedish Research Council VR (projects 2021-05311 and 2025-05450).
DS Repositorio Institucional de la Universidad de Huelva
RD 14 jul 2026