RT Journal Article
T1 Omics technologies and their applications to evaluate metal toxicity in mice M. spretus as a bioindicator
A1 García Sevillano, Miguel Ángel
A1 García Barrera, Tamara
A1 Abril, Nieves
A1 Pueyo, Carmen
A1 López Barea, Juan
A1 Gómez Ariza, José Luis
AB Metals are important components of living organisms since many biological functions criticallydepend on their interaction with some metal in the cell. However, human activities haveincreased toxic metal levels in the terrestrial andaquatic ecosystems affecting living organisms.The impact of metals on cellular metabolism and global homeostasis has been traditionallyassessed in free-living organisms by using conventional biomarkers; however, to obtain a globalvision of metal toxicity mechanisms and the responses that metals elicit in the organisms,new analytical methodologies are needed. We review the use of omics approaches to assess theresponse of living organisms under metal stress illustrating the possibilities of differentmethodologies on the basis of our previous results. Most of this research has been based onfree-living miceMus spretus, a conventional bioindicator used to monitor metal pollution inDoñana National Park (DNP) (SW Spain), which is an important European biological reserve formigrating birds affected by agricultural, mining and industrial activities. The benefits of usingomic techniques such as heterologous microarrays, proteomics methodologies (2-DE, iTRAQ®),metallomics, ionomics or metabolomics has been remarked; however, the complexity of theseareas requires the integration of omics to achieve a comprehensive assessment of theirenvironmental status. This article is part of a Special Issue entitled: Environmental and structuralproteomics.Biological SignificanceThis work presents new contributions in the study of environmental metal pollution interrestrial ecosystems using Mus spretus mice as bioindicator in Doñana National Park
PB Elsevier
SN 1874-3919
YR 2014
FD 2014
LK http://hdl.handle.net/10272/14509
UL http://hdl.handle.net/10272/14509
LA eng
NO García Sevillano, M.A., García Barrera, T., Abril, N., Pueyo, C., Lopez Barea, J., Gómez Ariza, J.L.: "Omics technologies and their applications to evaluate metal toxicity in mice M. spretus as a bioindicator". Journal of Proteomics. Vol. 104, págs. 4-23, (2014). DOI: 10.1016/j.jprot.2014.02.032
NO This work was supported by the project CTM2012-38720-C03-01 from the Spanish Ministry of Economy and Competitiveness, and by projects P08-FQM-3554, P09-FQM-4659, P08-CVI-03829, and P08-RNM-00523 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). M.A. Garcia Sevillano thanks the Ministerio de Educacion (Spain) for a PhD scholarship.
DS Repositorio Institucional de la Universidad de Huelva
RD 15 jun 2026