RT Journal Article
T1 Metabolomic screening of regional brain alterations in the APP/PS1 transgenic model of Alzheimer's disease by direct infusion mass spectrometry
A1 González Domínguez, Raúl
A1 García Barrera, Tamara
A1 Vitorica, Javier
A1 Gómez Ariza, José Luis
AB The identification of pathological mechanisms underlying to Alzheimer's disease is of great importance for the discovery of potential markers for diagnosis and disease monitoring. In this study, we investigated regional metabolic alterations in brain from the APP/PS1 mice, a transgenic model that reproduces well some of the neuropathological and cognitive deficits observed in human Alzheimer's disease. For this purpose, hippocampus, cortex, cerebellum and olfactory bulbs were analyzed using a high-throughput metabolomic approach based on direct infusion mass spectrometry. Metabolic fingerprints showed significant differences between transgenic and wild-type mice in all brain tissues, being hippocampus and cortex the most affected regions. Alterations in numerous metabolites were detected including phospholipids, fatty acids, purine and pyrimidine metabolites, acylcarnitines, sterols and amino acids, among others. Furthermore, metabolic pathway analysis revealed important alterations in homeostasis of lipids, energy management, and metabolism of amino acids and nucleotides. Therefore, these findings demonstrate the potential of metabolomic screening and the use of transgenic models for understanding pathogenesis of Alzheimer's disease.
PB Elsevier
SN 0731-7085
SN 1873-264X (electrónico)
YR 2015
FD 2015
LK http://hdl.handle.net/10272/14594
UL http://hdl.handle.net/10272/14594
LA eng
NO González Domínguez, R., García Barrera, T., Vitorica, J., Gómez Ariza, J.L.: "Metabolomic screening of regional brain alterations in the APP/PS1 transgenic model of Alzheimer's disease by direct infusion mass spectrometry". Journal of Pharmaceutical and Biomedical Analysis. Vol. 102, págs.  425-435, (2015). DOI: 10.1016/j.jpba.2014.10.009
NO This work was supported by the projects CTM2012-38720-C03-01 from the Ministerio de Ciencia e Innovacion and P008-FQM-3554 and P009-FQM-4659 from the Consejeria de Innovacion, Ciencia y Empresa (Junta de Andalucia). Raul Gonzalez Dominguez thanks the Ministerio de Educacion for a predoctoral scholarship.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026