@article{10272/22966,
year = {2017},
url = {https://hdl.handle.net/10272/22966},
abstract = {The present review focus on the analytical platforms and the workflow for toxicometabolomics with a
special emphasis on their strengths and pitfalls presenting as a case study the toxicometabolomics of
arsenic in mammals.
Although powerful analytical methods and techniques are currently available for metabolomics, the
main “bottleneck” is still the absence of unified protocols for sample preparation (e.g. quenching, solvents
used) as well as several important factors in toxicometabolomics, which drastically affect the
metabolism (e.g. selection of model organisms, xenobiotic doses, chemical form of the xenobiotic,
exposure route, biological sample). In this context, the applicability to complex samples, higher sensitivity,
specificity and the possibility to perform quantitative analysis offered by MS is crucial to probe
xenobiotic induced metabolic changes to evaluate the stress responses.
Nowadays, the use of different metabolomic platforms allowed determining important changes in the
metabolism induced by arsenic in mammals such as alterations in the energy (e.g. Glycolysis, Kreb's
cycle), amino acid, lipid, nucleotide and androgen metabolisms.},
organization = {This work was supported by the projects CTM2012-38720-C03-01 and CTM2015-67902-C-1-P from the Spanish Ministry of Economy and Competitiveness, and P12-FQM-0442 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). Gema Rodríguez Moro thanks the Spanish Ministry of Economy and Competitiveness and Belén Callejón Leblic thanks the Ministry of Education, Culture and Sport for predoctoral scholarships.},
publisher = {Elsevier},
title = {Mass spectrometry based analytical approaches and pitfalls for toxicometabolomics of arsenic in mammals: A tutorial review},
doi = {10.1016/j.aca.2017.10.019},
author = {García Barrera, Tamara and Rodríguez Moro, Gema and Callejón Leblic, María Belén and Arias Borrego, Ana and Gómez Ariza, José Luis},
}