RT Journal Article
T1 Mass spectrometry based analytical approaches and pitfalls for toxicometabolomics of arsenic in mammals: A tutorial review
A1 García Barrera, Tamara
A1 Rodríguez Moro, Gema
A1 Callejón Leblic, María Belén
A1 Arias Borrego, Ana
A1 Gómez Ariza, José Luis
AB The present review focus on the analytical platforms and the workflow for toxicometabolomics with aspecial emphasis on their strengths and pitfalls presenting as a case study the toxicometabolomics ofarsenic in mammals.Although powerful analytical methods and techniques are currently available for metabolomics, themain “bottleneck” is still the absence of unified protocols for sample preparation (e.g. quenching, solventsused) as well as several important factors in toxicometabolomics, which drastically affect themetabolism (e.g. selection of model organisms, xenobiotic doses, chemical form of the xenobiotic,exposure route, biological sample). In this context, the applicability to complex samples, higher sensitivity,specificity and the possibility to perform quantitative analysis offered by MS is crucial to probexenobiotic induced metabolic changes to evaluate the stress responses.Nowadays, the use of different metabolomic platforms allowed determining important changes in themetabolism induced by arsenic in mammals such as alterations in the energy (e.g. Glycolysis, Kreb'scycle), amino acid, lipid, nucleotide and androgen metabolisms.
PB Elsevier
SN 0003-2670
YR 2017
FD 2017
LK https://hdl.handle.net/10272/22966
UL https://hdl.handle.net/10272/22966
LA eng
NO García-Barrera, T., Rodríguez-Moro, G., Callejón-Leblic, B., Arias-Borrego, A., & Gómez-Ariza, J. L. (2018). Mass spectrometry based analytical approaches and pitfalls for toxicometabolomics of arsenic in mammals: A tutorial review. In Analytica Chimica Acta (Vol. 1000, pp. 41–66). Elsevier BV. https://doi.org/10.1016/j.aca.2017.10.019
NO This work was supported by the projects CTM2012-38720-C03-01 and CTM2015-67902-C-1-P from the Spanish Ministry of Economy and Competitiveness, and P12-FQM-0442 from the Regional Ministry of Economy, Innovation, Science and Employment (Andalusian Government, Spain). Gema Rodríguez Moro thanks the Spanish Ministry of Economy and Competitiveness and Belén Callejón Leblic thanks the Ministry of Education, Culture and Sport for predoctoral scholarships.
DS Repositorio Institucional de la Universidad de Huelva
RD 1 jun 2026