RT Journal Article
T1 Development of a metabolomic approach based on urine samples and direct infusion mass spectrometry
A1 González Domínguez, Raúl
A1 Castilla Quintero, Rocío
A1 García Barrera, Tamara
A1 Gómez Ariza, José Luis
AB The analysis of urine by direct infusion mass spectrometry suffers from ion suppression due to its high salt content and inter-sample variability caused by the differences in urine volume between persons. Thus, urine metabolomics requires a careful selection of the sample preparation procedure and a normalization strategy to deal with these problems. Several approaches were tested for metabolomic analysis of urine samples by direct infusion electrospray mass spectrometry (DI–ESI–MS), including solid phase extraction, liquid–liquid extraction, and sample dilution. In addition, normalization of results based on conductivity values and statistical treatment was performed to minimize sample variability. Both urine dilution and solid phase extraction with mixed mode sorbent considerably reduced the salt content in urine, providing comprehensive metabolomic fingerprints. Moreover, statistical data normalization enabled the correction of inter-sample physiological variability, improving the quality of results obtained. Therefore, high-throughput DI–ESI–MS fingerprinting of urine samples can be achieved with simple pretreatment procedures allowing the use of this noninvasive sampling in metabolomics. Finally, the optimized approach was tested in a pilot metabolomic investigation of urine samples from transgenic mice models of Alzheimer’s disease (APP/PS1) in order to illustrate the potential of the methodology.
PB Elsevier
SN 0003-2697
YR 2014
FD 2014
LK http://hdl.handle.net/10272/14476
UL http://hdl.handle.net/10272/14476
LA eng
NO González Domínguez, R., Castilla Quintero, R., García Barrera, T., Gómez Ariza, J.L.: "Development of a metabolomic approach based on urine samples and direct infusion mass spectrometry". Analytical Biochemistry Vol. 465, págs. 20-27, (2014). DOI: 10.1016/j.ab.2014.07.016
NO This work was supported by the project CTM2012-38720-C03-01 from the Ministerio de Ciencia e Innovacion and projects P012FQN-0442 and P009-FQM-4659 from the Consejeria de Innovacion, Ciencia y Empresa (Junta de Andalucia). Raul Gonzalez Dominguez thanks the Ministerio de Educacion for a predoctoral scholarship. The authors also thank Javier Vitorica (University of Seville) for providing transgenic mice.
DS Repositorio Institucional de la Universidad de Huelva
RD 28 may 2026