RT Journal Article T1 Composition of microalgae produced using different types of water and nutrient sources A1 Villaró Cos, Silvia A1 Cuaresma Franco, María A1 García Vaquero, Marco A1 Morán, Lara A1 Alarcón López, Francisco Javier A1 Lafarga Poyo, Tomás Valentín AB Microalgae are commonly referred to as "unicellular factories" due to their size and their capacity to produce and accumulate valuable biomolecules. The synthesis of a given biomolecule is generally triggered by external stimuli (e.g., light). This work evaluated the effect of different types of water and nutrient sources (freshwater, wastewater, fertilisers, or pig slurry) on the biochemical composition of the microalga Tetradesmus almeriensis produced on a pilot-scale. Overall, significant differences were observed in the biochemical composition of the biomasses (p < 0.05). The biomass produced using freshwater and fertilisers showed potential to be used as food given its high content of essential amino acids, carotenoids (mainly lutein and β-carotene), and PUFAs (C18:2 and C18:3). The microalgae produced using wastewater showed potential applications in the animal feed and agricultural industries. They showed a higher abundance of essential amino acids and carotenoids, including fucoxanthin and lycopene; the latter was only detected when the biomass was produced using secondary wastewater. The biomass produced using pig slurry as the source of nutrients showed potential for being used as animal feed or as an agricultural product given its high content in essential biomolecules and amino acids. The biochemical composition was not only a cause of different microbial diversities but also of the effect of the different composition of the culture medium that trigger different responses of the microalgal cells. PB Elsevier SN 2211-9264 YR 2024 FD 2024-01 LK https://hdl.handle.net/10272/23691 UL https://hdl.handle.net/10272/23691 LA eng NO Villaró-Cos, S., Cuaresma Franco, M., García-Vaquero, M., Morán, L., Alarcón, F. J., & Lafarga, T. (2024). Composition of microalgae produced using different types of water and nutrient sources. In Algal Research (Vol. 78, p. 103394). Elsevier BV. https://doi.org/10.1016/j.algal.2024.103394 NO This work forms part of the CLEAN⋅AIR (TED2021-131511A-I00)and SOLAR⋅FOODS (PID2022-136292OB-I00) projects, both funded bythe Spanish Ministry of Science and Innovation - MCIN/AEI/10.13039/501100011033 and the European Union NextGenerationEU/PRTR.This work was also funded by the BLUE⋅FUTURE (PCM_00083) project,funded by the Government of Andalusia and the European UnionNextGenerationEU/PRTR. Tom´as Lafarga would like to thank PPIT-UAL,Junta de Andalucía-FEDER 2021-2027 (Programme 54.A) and theRamon y Cajal Programme (RYC2021-031061-I) funded by MCIN/AEI/10.13039/501100011033 and European Union NextGenerationEU/PRTR. DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026