RT Journal Article T1 Simultaneous production of carotenoids and chemical building blocks precursors from chlorophyta microalgae A1 Rengel Domínguez, Rocío A1 Díaz Blanco, Manuel Jesús A1 Giráldez Díaz, Inmaculada A1 García Domínguez, María Trinidad A1 Vigara Fernández, Javier A1 León Bañares, Rosa María AB Replacement of fossil fuels has to be accompanied by the incorporation of bio-based procedures for the production of fine chemicals. With this aim, the microalga Chlamydomonas reinhardtii was selected for its ability to accumulate starch, an environmentally-friendly alternative source of chemical building blocks, such as 5′ -hydroxymethylfurfural or levulinic acid. The content of appreciated lipophilic coproducts was assessed in the selected microalga cultured at different nutritional conditions; and the parameters for the acidic hydrolysis of the algal biomass, obtained after pigments extraction, were optimized using a Central Composite Design. Response Surface Methodology predicted that the optimal hydrolysis conditions were elevated temperature, high DMSO % and short hydrolysis time for glucose. LA was favored at long times and high acid % and 5′ -HMF at lower acid % and high DMSO %. Chlamydomonas can therefore be used as a sustainable feedstock for the simultaneous production of high-added value lipophilic compounds and platform chemicals. PB Elsevier SN 0960-8524 YR 2022 FD 2022-03-18 LK http://hdl.handle.net/10272/21161 UL http://hdl.handle.net/10272/21161 LA eng NO Rengel, R., Giraldez, I., Díaz, M. J., García, T., Vigara, J., & León, R. (2022). Simultaneous production of carotenoids and chemical building blocks precursors from chlorophyta microalgae. Bioresource Technology, 351, 127035. https://doi.org/10.1016/j.biortech.2022.127035 NO Funding for open access charge: Universidad de Huelva / CBUA. DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026