RT Journal Article
T1 Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology
A1 Montero Lobato, Zaida
A1 Ramos Merchante, Adrián
A1 Fuentes Cordero, Juan Luis
A1 Sayago Gómez, Ana
A1 Fernández Recamales, María Ángeles
A1 Martínez Espinosa, María Rosa
A1 Vega Piqueres, José María
A1 Vílchez Lobato, Carlos
A1 Garbayo Nores, Inés
AB Haloferax mediterranei produces C50 carotenoids that have strong antioxidant properties.The response surface methodology (RSM) tool helps to accurately analyze the most suitable conditionsto maximize C50 carotenoids production by haloarchaea. The effects of temperature (15–50ºC),pH (4-10), and salinity (5–28% NaCl (w/v)) on the growth and carotenoid content of H. mediterraneiwere analyzed using the RSM approach. Growth was determined by measuring the turbidityat 600 nm. To determine the carotenoid content, harvested cells were lysed by freeze/thawing,then re-suspended in acetone and the total carotenoid content determined by measuring theabsorbance at 494 nm. The analysis of carotenoids was performed by an HPLC system coupled withmass spectrometry. The results indicated the theoretical optimal conditions of 36.51 or 36.81ºC, pH of8.20 or 8.96, and 15.01% or 12.03% (w/v) salinity for the growth of haloarchaea (OD600 = 12.5 +- 0.64)and production of total carotenoids (3.34 +- 0.29 mg/L), respectively. These conditions were validatedexperimentally for growth (OD600 = 13.72 +- 0.98) and carotenoid production (3.74 +- 0.20 mg/L).The carotenoid profile showed four isomers of bacterioruberin (89.13%). Our findings suggest thatthe RSM approach is highly useful for determining optimal conditions for large-scale production ofbacterioruberin by haloarchaea.
PB National Center for Biotechnology Information
SN 1660-3397
YR 2018
FD 2018-10
LK http://hdl.handle.net/10272/16052
UL http://hdl.handle.net/10272/16052
LA eng
NO Zaida Montero-Lobato, Adrián Ramos-Merchante, Juan Luis Fuentes, Ana Sayago, Ángeles Fernández-Recamales, Rosa María Martínez-Espinosa, José María Vega, Carlos Vílchez,Inés Garbayo. Optimization of Growth and Carotenoid Production by Haloferax mediterranei Using Response Surface Methodology. Mar Drugs. 2018 Oct 9;16(10). ISSN: 1660-3397. DOI : 10.3390/md16100372.
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026