RT Journal Article T1 Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana A1 León Vaz, Antonio A1 León Bañares, Rosa María A1 Díaz-Santos, Encarnación A1 Vigara Fernández, Javier A1 Raposo, Sara AB There has been growing interest in the use of microalgae for the production of biofuels, but production costs continue to be too high to compete with fossil fuel prices. One of the main limitations for photobioreactor productivity is light shielding, especially at high cell densities. The growth of the green microalga Chlorella sorokiniana, a robust industrial species, has been evaluated under different trophic conditions with traditional carbon sources, such as glucose and sucrose, and alternative low cost carbon sources, such as carob pod extract, industrial glycerol and acetate-rich oxidized wine waste lees. The mixotrophic cultivation of this microalga with wine waste lees alleviated the problems of light shielding observed in photoautotrophic cultures, improving specific growth rate (0.052 h-1 ) compared with the other organic sources. The fed-batch mixotrophic culture of Chlorella sorokiniana in a 2 L stirred tank reactor, with optimized nutritional conditions, 100 mM of acetate coming from the oxidized wine waste lees and 30 mM of ammonium, produced an algal biomass concentration of 11 gL-1 with a lipid content of 38% (w/w). This fed-batch strategy has been found to be a very effective means to enhance the biomass and neutral lipid productivity SN 1871-6784 YR 2019 FD 2019-02-06 LK http://hdl.handle.net/10272/21176 UL http://hdl.handle.net/10272/21176 LA eng NO León-Vaz, A., León, R., Díaz-Santos, E., Vigara, J., & Raposo, S. (2019). Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana. In New Biotechnology (Vol. 51, pp. 31–38). Elsevier BV. https://doi.org/10.1016/j.nbt.2019.02.001 DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026