RT Book, Section T1 Enhancement of carotenoid production in Nannochloropsis by phosphate and sulphur limitation A1 Forján Lozano, Eduardo A1 Garbayo Nores, Inés A1 Casal Bejarano, Carlos A1 Vílchez Lobato, Carlos AB Because of its value as antioxidants, food-supplements and in animal-feeding, carotenoids are among themost attractive target compounds of marine microalgae. In this contribution the influence of essentialnutrients (N, S and P) limitation on the accumulation of carotenoids in the marine microalgaNannochloropsis gaditana has been studied. High and rapid biomass production, which depends ongrowth conditions, is the first step in a bioproduction process. In our work was observed thatNannochloropsis gaditana growth was significantly faster when using 5 % (v/v) CO2 in air instead air orHCO3- as carbon source, at two different pH values assayed (7.2 and 8.2), as revealed from cellchlorophyll content, nitrate consumption rates and light-dependent oxygen production. Afterwards, cellsgrown to exponential phase were incubated in culture media containing none or limiting concentrations ofeither nitrate, sulphate or phosphate. The results showed that both starvation and low concentrations ofnutrients (0.005 mM P, 0.2 mM N, 1.1 · 10-5 mM S) drove to increases of carotenoids/chlorophyll ratios,specially for sulphur limitation. In a general view, zeaxanthin and violaxanthin content increased in alllimiting conditions tested, the increase of violaxanthin content being less pronounced. PB Formatex YR 2007 FD 2007 LK http://hdl.handle.net/10272/4510 UL http://hdl.handle.net/10272/4510 LA spa NO FORJÁN LOZANO, E., GARBAYO NORES, I., CASAL BEJARANO, C., VÍLCHEZ LOBATO, C. (2007). "Enhancement of carotenoid production in Nannochloropsis by phosphate and sulphur limitation". En: Communicating Current Research and Educational Topics and Trends in Applied Microbiology. Microbiology Book Series, VOL. I, pp. 356-364. Formatex. Badajoz, España. ISBN-13: 978-84-611-9421-6(SERIE), ISBN-13: 978-84-611-9422-3(VOL. I). DS Repositorio Institucional de la Universidad de Huelva RD 30 may 2026