RT Journal Article
T1 A novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cells
A1 Pérez, David
A1 Bautista Pulido, María
A1 León Bañares, Rosa María
A1 Molina Márquez, Ana María
A1 Vila Spinola, Marta
A1 López López, Manuel
A1 López Cornejo, Pilar
AB The design and preparation of new vectors to transport genetic material and increase the transfection efficiencycontinue being an important research line. Here, a novel biocompatible sugar-based polymer derived from Dmannitolhas been synthesized to be used as a gene material nanocarrier in human (gene transfection) andmicroalga cells (transformation process). Its low toxicity allows its use in processes with both medical and industrialapplications. A multidisciplinary study about the formation of polymer/p-DNA polyplexes has beencarried out using techniques such as gel electrophoresis, zeta potential, dynamic light scattering, atomic forcemicroscopy, and circular dichroism spectroscopy. The nucleic acids used were the eukaryotic expression plasmidpEGFP-C1 and the microalgal expression plasmid Phyco69, which showed different behaviors. The importance ofDNA supercoiling in both transfection and transformation processes was demonstrated. Better results were obtainedin microalga cells nuclear transformation than in human cells gene transfection. This was related to theplasmid’s conformational changes, in particular to their superhelical structure. It is noteworthy that the samenanocarrier has been used with eukaryotic cells from both human and microalga.
PB Elsevier
SN 0927-7765
SN 1873-4367 (electrónico)
YR 2023
FD 2023-02
LK https://hdl.handle.net/10272/22444
UL https://hdl.handle.net/10272/22444
LA eng
NO Pérez, D., Moyá, M. L., Bautista, M., León, R., Molina-Márquez, A., Vila, M., Romero-Azogil, L., Benito, E., de Gracia García-Martín, M., Moreno-Gordillo, P., Rosado, I. V., Balestra, F. R., Huertas, P., López-López, M., & López-Cornejo, P. (2023). A novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cells. In Colloids and Surfaces B: Biointerfaces (Vol. 224, p. 113219). Elsevier BV. https://doi.org/10.1016/j.colsurfb.2023.113219
NO This work was supported by the Consejería de Conocimiento, Innovación y Universidades de la Junta de Andalucía (FQM-206, FQM-274, FQM-135 and P20–01234); VI Plan Propio Universidad de Sevilla (PP2019/00000748), and the European Union (Feder Funds).
DS Repositorio Institucional de la Universidad de Huelva
RD 31 may 2026