RT Journal Article T1 Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production A1 Fuentes Cordero, Juan Luis A1 Montero Lobato, Zaida A1 Cuaresma Franco, María A1 Ruiz Domínguez, María del Carmen A1 Mogedas Romero, Benito A1 Garbayo Nores, Inés A1 González del Valle, Manuel A1 Vílchez Lobato, Carlos A1 Ruiz Domínguez, María del Carmen AB The large-scale biomass production is an essential step in the biotechnological applicationsof microalgae. Coccomyxa onubensis is an acidophilic microalga isolated from the highly acidic watersof Río Tinto (province of Huelva, Spain) and has been shown to accumulate a high concentrationof lutein (9.7 mg g-1dw), a valuable antioxidant, when grown at laboratory-scale. A productivityof 0.14 g L-1 d-1 was obtained by growing the microalga under outdoor conditions in an 800 Ltubular photobioreactor. The results show a stable biomass production for at least one month andwith a lutein content of 10 mg g-1dw, at pH values in the range 2.5–3.0 and temperature in the range10–25 ºC. Culture density, temperature, and CO2 availability in highly acidic medium are rate-limitingconditions for the microalgal growth. These aspects are discussed in this paper in order to improvethe outdoor culture conditions for competitive applications of C. onubensis. PB MDPI SN 2227-9717 YR 2020 FD 2020-03 LK http://hdl.handle.net/10272/17998 UL http://hdl.handle.net/10272/17998 LA eng NO Fuentes, J.L., Montero, Z., Cuaresma Franco, M., Ruiz Domínguez, M.C., Mogedas Romero, B., Garbayo Nores, I., González del Valle, M., Vílchez Lobato, C. (2020). Outdoor Large-Scale Cultivation of the Acidophilic Microalga Coccomyxa onubensis in a Vertical Close Photobioreactor for Lutein Production. Processes, 8(3), 324. DOI: https://doi.org/10.3390/pr8030324 NO Authors want to thank the PhD-Grant (2015/7949) from CEIMAR (Marine International Campus ofExcellence, Spain) to Juan Luis Fuentes. DS Repositorio Institucional de la Universidad de Huelva RD 14 jul 2026