Toward Two-Photon Absorbing Dyes with Unusually Potentiated Nonlinear Fluorescence Response

Benítez Martín, CarlosLi, ShimingDomínguez Alfaro, AntonioPischel, Uwe2020-10-082020-10-082020-08Benítez Martín, C., Li, S., Domínguez Alfaro, A., ... Pischel, U. (2020). Toward Two-Photon Absorbing Dyes with Unusually Potentiated Nonlinear Fluorescence Response. Journal of the American Chemical Society, 142(35), 14854–14858. DOI: https://doi.org/10.1021/jacs.0c073770002-7863http://hdl.handle.net/10272/18824The combination of two two-photon-induced processes in a Förster resonance energy transfer (FRET)-operated photochromic fluorene-dithienylethene dyad lays the foundation for the observation of a quartic dependence of the fluorescence signal on the excitation light intensity. While this photophysical behavior is predicted for a four-photon absorbing dye, the herein proposed approach opens the way to use two-photon absorbing dyes, reaching the same performance. Hence, the spatial resolution limit, being a critical parameter for applications in fluorescence imaging or data storage with common two-photon absorbing dyes, is dramatically improved.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/Dyes and pigmentsFluorescenceFluorescence resonance energy transferAbsorption spectroscopyLasersToward Two-Photon Absorbing Dyes with Unusually Potentiated Nonlinear Fluorescence Responsejournal article10.1021/jacs.0c07377open access