Morón Ortiz, ÁngelesMapelli Brahm, PaulaLeón Vaz, AntonioBenítez González, Ana M.León Bañares, Rosa MaríaMeléndez Martínez, Antonio J.2024-02-022024-02-022024Morón-Ortiz, Á., Mapelli-Brahm, P., León-Vaz, A., Benitez-González, A. M., León, R., & Meléndez-Martínez, A. J. (2024). Ultrasound-assisted extraction of carotenoids from phytoene-accumulating Chlorella sorokiniana microalgae: Effect of milling and performance of the green biosolvents 2-methyltetrahydrofuran and ethyl lactate. In Food Chemistry (Vol. 434, p. 137437). Elsevier BV. https://doi.org/10.1016/j.foodchem.2023.1374370162-32571573-3432 (electrónico)https://hdl.handle.net/10272/23086This study aimed at optimizing the accumulation of phytoene in Chlorella sorokiniana by using norflurazon and investigating the capacity of green and traditional solvents to extract carotenoids by ultrasound-assisted extraction with and without previous milling. Phytoene-rich first described C. sorokiniana biomass was used, both fresh, freeze-dried, and encapsulated. The ideal dose of norflurazon (1 µg/mL) was selected to block the carotenoid pathway at the level of phytoene desaturase and induce the accumulation of phytoene in C. sorokiniana. A mill pre-treatment allowed a higher recovery of carotenoids compared to non-milled samples, in both the freeze-dried and encapsulated matrices. 2-Methyloxolane provided a higher total carotenoid content (4.75–5546.96 µg/g) compared to the other solvents tested in all the matrices, proving a promising bio-based solvent to replace traditional organic ones for the extraction of microalgal carotenoids.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/Bioactive compoundsNorflurazon2-methyloxolanePowderingSonicationSustainabilityjournal article10.1016/j.foodchem.2023.137437open access23 Química