Rubiales Gómez, Jaime S.Castro Gutiérrez, JairoRodríguez García, CarlosSarmiento Carbajal, JésicaCabrera Castro, Remedios2026-03-232026-03-232026Rubiales-Gómez, J. S., Castro-Gutiérrez, J., Rodríguez-García, C., Sarmiento-Carbajal, J., & Cabrera-Castro, R. (2026). Differentiation of Lesueurigobius sanzi and L. suerii through otolith morphometric analysis. Estuarine, Coastal and Shelf Science, 332, 109763. https://doi.org/10.1016/j.ecss.2026.1097631096-0015https://hdl.handle.net/10272/28128Otolith morphology is a valuable tool for species identification, particularly for morphologically similar species. This study evaluates the potential of otolith morphometry to distinguish Lesueurigobius sanzi and L. suerii, two closely related species commonly found as fishery discards in the Gulf of Cádiz. A total of 1,019 individuals (L. sanzi: 921, L. suerii: 98) were collected and their otoliths were analysed using the R package "shapeR" to obtain morphometric parameters and standardised wavelet coefficients. Statistical analyses revealed significant morphological differences between the species. Principal Component Analysis (PCA) showed that otolith length, perimeter, and shape indices contribute significantly to species differentiation. Furthermore, a Linear Discriminant Analysis (LDA) based on standardised wavelet coefficients demonstrated high classification accuracy (91.6%), confirming the effectiveness of this approach for species identification. These findings highlight the reliability of otolith morphometry as a robust method for distinguishing L. sanzi and L. suerii, providing valuable insights for fisheries management and biodiversity studies. This research contributes to the development of classification methods, essential for sustainable fishery assessments and ecological research.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Morphometric analysisFishery discardsSpecies classificationMorphological differentiationSagittal otolithsWavelet coefficientsjournal article10.1016/j.ecss.2026.109763open access3105 Peces y Fauna Silvestre2401.14-4 Taxonomía Animal. Peces