Impact of Antibiotic-Induced Depletion of Gut Microbiota and Selenium Supplementation on Plasma Selenoproteome and Metal Homeostasis in a Mice Model

Callejón Leblic, María BelénSelma Royo, MartaCollado, Maria CarmenAbril, NievesGarcía Barrera, Tamara2023-03-242023-03-242021Callejón-Leblic, B., Selma-Royo, M., Collado, M. C., Abril, N., & García-Barrera, T. (2021). Impact of Antibiotic-Induced Depletion of Gut Microbiota and Selenium Supplementation on Plasma Selenoproteome and Metal Homeostasis in a Mice Model. In Journal of Agricultural and Food Chemistry (Vol. 69, Issue 27, pp. 7652–7662). American Chemical Society (ACS). https://doi.org/10.1021/acs.jafc.1c026220021-85611520-5118 (electrónico)https://hdl.handle.net/10272/21856Determinación de metales y Selenoproteinas y su interacción con la Microbiota intestinal tras la ingesta de suplementos de selenio y la administración de antibióticos a ratones Mus musculus.Selenium (Se) is a micronutrient involved in important health functions and it has been suggested to shape gut microbiota. Limited information on Se assimilation by gut microbes and the possible link with selenoproteins are available. For this purpose, conventional and gut microbiota-depleted BALB/c mice were fed a Se-supplemented diet. The absolute quantification of mice plasma selenoproteins was performed for the first time using heteroatom-tagged proteomics. The gut microbiota profile was analyzed by 16S rRNA gene sequencing. Se-supplementation modulated the concentration of the antioxidant glutathione peroxidase and the Se-transporter selenoalbumin as well as the metal homeostasis, being influenced by microbiota disruption, which suggests an intertwined mechanism. Se also modulated microbiota diversity and richness and increased the relative abundance of some health- relevant taxa (e.g., families Christensenellaceae, Ruminococcaceae, and Lactobacillus genus). This study demonstrated the potential beneficial effects of Se on gut microbiota, especially after antibiotic-treatment and the first associations between specific bacteria and plasma selenoproteins.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/SelenoproteinasMicrobiotaSuplementacionSelenioChemical speciationHeteroatom-tagged proteomicsICP-MSImpact of Antibiotic-Induced Depletion of Gut Microbiota and Selenium Supplementation on Plasma Selenoproteome and Metal Homeostasis in a Mice Modeljournal article10.1021/acs.jafc.1c02622open access23 Química2301 Química Analítica