Guimarães, Bárbara O.Villarreal Toribio, BelénGarcía Barrera, TamaraArias Borrego, AnaGremmen, PieterWijffels, René H.Barbosa, Maria J.D'Adamo, Sarah2023-03-032023-03-032022Guimarães, B. O., Villarreal-Toribio, B., García-Barrera, T., Arias-Borrego, A., Gremmen, P., Wijffels, R. H., Barbosa, M. J., & D’Adamo, S. (2022). Effect of sulphur on selenium accumulation and speciation in Nannochloropsis oceanica. In Journal of Functional Foods (Vol. 96, p. 105215). Elsevier BV. https://doi.org/10.1016/j.jff.2022.1052151756-4646https://hdl.handle.net/10272/21706Sulphur (S) and selenium (Se) are chemically similar. Once Se is taken up, it substitutes S in S-containing amino acids. This study investigated the effect of S on selenite accumulation in the microalga Nannochloropsis oceanica. [0–28 mM] S concentrations and selenite concentrations of 0 and 30 µM were tested. S concentrations of = 3 mM led to decreased cell growth whereas cultures with = 4 mM were not growth limited. Se accumulation increased up to 8-fold when using S = 2 mM and decreased with S 28 mM. The average relative abundance of organic Se species was selenomethionine (SeMet) 98.2 %, selenocystine (SeCys2) 1.4 % and selenomethyl selenocysteine (SeMeSeCys) 0.4 %. Total fatty acids were not affected by S limitation or Se presence. This is the first study on the effect of S on selenite accumulation, organic Se speciation of N. oceanica and its potential as an organic Se-enriched food/feed ingredient.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/Nannochloropsis oceanicaSeleniumSeleniteAquacultureSe speciationChemical similarityjournal article10.1016/j.jff.2022.105215open access23 Química