Blázquez Carmona, PabloRuiz Mateos, RaquelBarrasa Fano, JorgeShapeti, ApekshaMartín Alfonso, José EnriqueDomínguez Abascal, JaimeVan Oosterwyck, HansReina Romo, EstherSanz Herrera, José A.2025-01-102025-01-102024-07Blázquez-Carmona, P., Ruiz-Mateos, R., Barrasa-Fano, J., Shapeti, A., Martín-Alfonso, J. E., Domínguez, J., Van Oosterwyck, H., Reina-Romo, E., & Sanz-Herrera, J. A. (2024). Quantitative atlas of collagen hydrogels reveals mesenchymal cancer cell traction adaptation to the matrix nanoarchitecture. In Acta Biomaterialia (Vol. 185, pp. 281–295). Elsevier BV. https://doi.org/10.1016/j.actbio.2024.07.0021742-70611878-7568 (electrónico)https://hdl.handle.net/10272/24759Collagen-based hydrogels are commonly used in mechanobiology to mimic the extracellular matrix. A quantitative analysis of the influence of collagen concentration and properties on the structure and me- chanics of the hydrogels is essential for tailored design adjustments for specific in vitro conditions. We combined focused ion beam scanning electron microscopy and rheology to provide a detailed quanti- tative atlas of the mechanical and nanoscale three-dimensional structural alterations that occur when manipulating different hydrogel’s physicochemistry. Moreover, we study the effects of such alterations on the phenotype of breast cancer cells and their mechanical interactions with the extracellular matrix. Regardless of the microenvironment’s pore size, porosity or mechanical properties, cancer cells are able to reach a stable mesenchymal-like morphology. Additionally, employing 3D traction force microscopy, a positive correlation between cellular tractions and ECM mechanics is observed up to a critical threshold, beyond which tractions plateau. This suggests that cancer cells in a stable mesenchymal state calibrate their mechanical interactions with the ECM to keep their migration and invasiveness capacities unaltered.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/MechanobiologyBreast cancer cellsTraction force microscopyTumor microenvironmentCell morphologyFIB-SEMjournal article10.1016/j.actbio.2024.07.002open access32 Ciencias Médicas2302 Bioquímica