González Domínguez, RaúlGonzález Domínguez, ÁlvaroFernández Recamales, María Ángeles2020-06-152020-06-152020-06González Domínguez, R., González Domínguez, Á., Sayago, A., & Fernández Recamales, Á. (2020). Recommendations and Best Practices for Standardizing the Pre-Analytical Processing of Blood and Urine Samples in Metabolomics. Metabolites, 10(6), 229. DOI: https://doi.org/10.3390/metabo100602292218-1989http://hdl.handle.net/10272/18307Metabolomics can be significantly influenced by a range of pre-analytical factors, such as sample collection, pre-processing, aliquoting, transport, storage and thawing. This therefore shows the crucial need for standardizing the pre-analytical phase with the aim of minimizing the inter-sample variability driven by these technical issues, as well as for maintaining the metabolic integrity of biological samples to ensure that metabolomic profiles are a direct expression of the in vivo biochemical status. This review article provides an updated literature revision of the most important factors related to sample handling and pre-processing that may affect metabolomics results, particularly focusing on the most commonly investigated biofluids in metabolomics, namely blood plasma/serum and urine. Finally, we also provide some general recommendations and best practices aimed to standardize and accurately report all these pre-analytical aspects in metabolomics research.engAtribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/MetabolomicsPre-analyticsBloodUrineCollectionProcessingStoragejournal article10.3390/metabo10060229open access