Red Light Enhances Biomass and Bioactive Compounds Through Photosynthetic Acclimation in Anabaena variabilis

Abstract

Light irradiance and spectral quality are key environmental factors that influence the growth, photosynthetic performance, and metabolic responses of cyanobacteria. In this study, the effects of increasing white and PAR-red light irradiances on Anabaena variabilis were evaluated in repeated-batch cultures, focusing on photosynthetic efficiency, biomass productivity, and the modulation of antioxidant systems, while cultures maintained under constant irradiance were used as control. Results showed that A. variabilis can maintain photosynthetic efficiency, as indicated by FV/FM values, within the optimal range for healthy cultures despite variations in light conditions. PAR-red light, in particular, enhanced biomass productivity and induced stronger photoacclimation responses compared to white light. Moreover, analysis of chlorophyll fluorescence (JIP parameters) revealed that photosynthetic machinery adapts to increased irradiance by modulating energy fluxes. Dissipated energy (DI0/RC) increases by 4.5-fold under increasing PAR-red light with respect to control cultures, which suggests that PAR-red light promotes thermal dissipation of excess absorbed energy at the phycobilisome level, independently of and complementarily to, the increase in light-harvesting antenna pigments (chlorophylls and phycobiliproteins), thereby reducing the net oxidative pressure in the electron transport chain. The increase in photosynthetic pigments reflects an adaptive adjustment to optimize light harvesting under red light, with a phycocyanin content of 123 mg·g−1 biomass, 30% higher than that obtained in control culture. Overall, A. variabilis demonstrated a robust capacity to acclimate increasing light irradiance and varying light quality through coordinated photoacclimation and antioxidant responses, in repeated-batch cultures. These findings highlight its physiological flexibility, which can be properly driven to maximize the production of valuable bioactive compounds, particularly phycobiliproteins such as phycocyanin, with applications in biotechnology.