UV-A mediated modulation of photosynthetic efficiency, xanthophyll cycle and fatty acid production of Nannochloropsis

Abstract

Nannochloropsis, a green microalga, is source for commercially valuable compounds as extensively described and, in particular, is recognized as a good potential source of EPA (20:5v3), an important polyunsaturated fatty acid for human consumption for prevention of several diseases. Climate change might include variation in the UV levels, as one of the consequences derived from the antropogenic activity. This paper shows the response of Nannochloropsis cultures exposed for 7 days to UV-A added to PAR. Growth rates and photosynthetic activity were assessed to determine the impact of UV-A increased levels on the cell growth and basic metabolism activity. Xanthophyll pigments (zeaxanthin and violaxanthin), carotenoids (cantaxanthin and β-carotene) and PUFAs (miristic, palmitic, palmitoleic, araquidonic and eicosapentanoic acids) were measured for assessing the antioxidant response of the microalgae to added UV-A radiation to PAR. The results show that the modulated use of UV-A radiations can led to increased growth rates which are sustained in time by an increased light transduction activity. The expected antioxidant response to the incident UV-A radiation consisted of increases in zeaxanthin and β-carotene contents –synthesis of antioxidant carotenoids- and increases in the SFAs (saturated fatty acids) to PUFAs (polyunsaturated fatty acids) ratio. The results suggest that modulated UV-A radiation can be used as a tool to stimulate value molecules accumulation in microalgae through an enhanced both light transduction process and antioxidant response, while sustaining cell growth