Simple and Efficient Green Extraction of Steviol Glycosides from Stevia rebaudiana Leaves

Abstract

The food industry has currently shown great interest in alternative sweeteners to sugars with the aim of producing healthier products. In light of this, steviol glycosides are natural low-caloric sweeteners present in Stevia rebaudiana, which have additionally been described as bioactive components with potential therapeutic properties. In this work, a green method for the extraction of steviol glycosides from stevia leaves was optimized by applying a factorial screening design of five variables (time, temperature, agitation, grinding, and sample–solvent ratio) and the subsequent response surface design of Box-Behnken. The optimized extraction method allows for the recovery of stevia sweeteners in a simple and e cient manner by using tap water as the extractant, without the application of an auxiliary energy source to reduce costs, thus representing an interesting strategy for their industrial-scale production.