Reversible Energy-Transfer Switching on a DNA Scaffold

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dc.contributor.authorBälter, Magnus
dc.contributor.authorHammarson, Martin
dc.contributor.authorRemón Ruiz, Patricia
dc.contributor.authorLi, Shiming
dc.contributor.authorGale, Nittaya
dc.contributor.authorBrown, Tom
dc.contributor.authorAndréasson, Joakim
dc.date.accessioned2024-02-07T11:59:28Z
dc.date.available2024-02-07T11:59:28Z
dc.date.issued2015-02
dc.description.abstractWe show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.es_ES
dc.description.departmentQuímica "Profesor José Carlos Vílchez Martín"
dc.description.sponsorshipThis work was financed by the Swedish Research Council VR (grant 622-2010-280), the European Research Council (ERC FP7/2007-2013 grant no. 203952), and the BBSRC (sLOLA grant BB/J001694/1 "extending the boundaries of nucleic acid chemistry”). The authors thank Melina Gilbert Gatty for assistance with the SPC measurements and Niklas Bosaeus for running the electrophoresis gels.es_ES
dc.identifier.citationBälter, M., Hammarson, M., Remón, P., Li, S., Gale, N., Brown, T., & Andréasson, J. (2015). Reversible Energy-Transfer Switching on a DNA Scaffold. In Journal of the American Chemical Society (Vol. 137, Issue 7, pp. 2444–2447). American Chemical Society (ACS). https://doi.org/10.1021/ja512416nes_ES
dc.identifier.doi10.1021/ja512416n
dc.identifier.issn0002-7863
dc.identifier.issn1520-5126 (electrónico)
dc.identifier.urihttps://hdl.handle.net/10272/23150
dc.language.isoenges_ES
dc.publisherAmerican Chemical Society (ACS)es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.rights.accessRightsopen accesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es/*
dc.subject.otherChromophoreses_ES
dc.subject.otherFluorescence resonance energy transferes_ES
dc.subject.otherGeneticses_ES
dc.subject.otherIsomerizationes_ES
dc.subject.otherScaffoldses_ES
dc.subject.unesco23 Químicaes_ES
dc.titleReversible Energy-Transfer Switching on a DNA Scaffoldes_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoR
dspace.entity.typePublication
relation.isAuthorOfPublication48a77d6a-af6c-4e1b-9e9a-65dbe12a6363
relation.isAuthorOfPublication.latestForDiscovery48a77d6a-af6c-4e1b-9e9a-65dbe12a6363

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