Reversible Energy-Transfer Switching on a DNA Scaffold

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2015 J Am Chem Soc.pdf (510.06 KB)

Publication date

2015-02

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Química "Profesor José Carlos Vílchez Martín"

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Center

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https://hdl.handle.net/10272/23150

DOI

10.1021/ja512416n

Abstract

We show that FRET between Pacific Blue (PB) and Alexa488 (A488) covalently attached to a DNA scaffold can be reversibly controlled by photochromic switching of a spiropyran derivative. With the spiropyran in the closed spiro isomeric form, FRET occurs freely between PB and A488. UV-induced isomerization to the open merocyanine form shuts down the FRET process by efficient quenching of the PB excited state. The process is reversed by exposure to visible light, triggering the isomerization to the spiro isomer.

Keywords

Chromophores; Fluorescence resonance energy transfer; Genetics; Isomerization; Scaffolds

Unesco Subjects

23 Química

Bibliographic citation

Bälter, M., Hammarson, M., Remón, P., Li, S., Gale, N., Brown, T., & Andréasson, J. (2015). Reversible Energy-Transfer Switching on a DNA Scaffold. In Journal of the American Chemical Society (Vol. 137, Issue 7, pp. 2444–2447). American Chemical Society (ACS). https://doi.org/10.1021/ja512416n

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